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KMID : 0358319930340040741
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Korean Journal of Urology
1993 Volume.34 No. 4 p.741 ~ p.747
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Clinical Significance of Silver Binding Nucleolar Organizer Regions(AgNORs) in Transitional Cell Carcinoma of he Bladder: the Correlative Study with Flow Cytometric SPF, PI and PCNA
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박홍석
천 준/한정호/김한겸/김제종/고성건
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Abstract
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Nucleolar organizer regions(NORs) contain coding genes for ribosomal RNA and contribute the regulation of cellular protein synthesis. AgNORs numbers correlated with growth fraction and have been reported the AgNORs counts may have a diagnostic
and
prognostic utility in other human tumors. We investigated the diagnostic usefulness of AgNORs staining technique as a discriminant for malignancy and assessed the value as a potential method for the estimation of cell kinetics. In addition, we
compared
the AgNOR counts with flow cytometric analysis of ploidy, S-phase fraction, proliferation index, and PCNA expression rate. There was a statistically significant difference of AgNORs counts between superficial bladder tumor and invasive bladder
tumor.
But there was no relationship between the mean number of AgNORs per nucleus and histological grade. DNA aneuploid group was associated with higher AgNORs counts than diploid group, but the difference was statistically insignificant. The mean
number
o
AgNORs per nucleous had significant relationship to SPF(r=0.43, p<0.05) and PI(r=0.41, p<0.05).
We concluded that this method alone does not offer a reliable histological discriminant for malignancy. Further studies are needed to confirm that AgNORs counting is a useful method for evaluating the proliferative activity and this technique may
serve
as a prognostic factor additonal to the current histopathological grading criteria of the bladder cancer.
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KEYWORD
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